To determine the elution order of compounds from an HPLC column containing a reversed-phase packing, we need to consider their polarity. In reversed-phase HPLC, the stationary phase is nonpolar, while the mobile phase is polar.

In this case, benzene is the most polar compound among the three options. It has a higher affinity for the polar mobile phase and will be eluted first.

Next, we have diethyl ether, which is less polar than benzene but more polar than n-hexane. It will be eluted after benzene.

Finally, n-hexane is the least polar compound among the three. It has a higher affinity for the nonpolar stationary phase and will be eluted last.

Therefore, the elution order from the HPLC column would be: benzene, diethyl ether, n-hexane.

Question

To determine the elution order of compounds from an HPLC column containing a reversed-phase packing, we need to consider their polarity. In reversed-phase HPLC, the stationary phase is nonpolar, while the mobile phase is polar.

In this case, benzene is the most polar compound among the three options. It has a higher affinity for the polar mobile phase and will be eluted first.

Next, we have diethyl ether, which is less polar than benzene but more polar than n-hexane. It will be eluted after benzene.

Finally, n-hexane is the least polar compound among the three. It has a higher affinity for the nonpolar stationary phase and will be eluted last.

Therefore, the elution order from the HPLC column would be: benzene, diethyl ether, n-hexane.

Knowee AI · Accepted Answer

To determine the elution order of compounds from an HPLC column containing a reversed-phase packing, we need to consider their polarity. In reversed-phase HPLC, the stationary phase is nonpolar, while the mobile phase is polar.

In this case, benzene is the most polar compound among the three options. It has a higher affinity for the polar mobile phase and will be eluted first.

Next, we have diethyl ether, which is less polar than benzene but more polar than n-hexane. It will be eluted after benzene.

Finally, n-hexane is the least polar compound among the three. It has a higher affinity for the nonpolar stationary phase and will be eluted last.

Therefore, the elution order from the HPLC column would be: benzene, diethyl ether, n-hexane.

Indicate the order in which the following compounds would be eluted from an HPLC columncontaining a reversed-phase packing: benzene, diethyl ether, n-hexane

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