In what direction is the strand of DNA read by RNA Polymerase II? 5' to 3' 3' to 3' 3' to 5' 5' to 5' Varies between species
Question
In what direction is the strand of DNA read by RNA Polymerase II?
5' to 3'3' to 3'3' to 5'5' to 5'- Varies between species
Solution
Answer
RNA Polymerase II reads the DNA strand in the 3' to 5' direction. This is necessary because RNA Polymerase synthesizes the RNA strand in the 5' to 3' direction. During transcription, the enzyme unwinds the DNA and constructs a complementary RNA strand based on the template DNA strand's nucleotide sequence.
Thus, while the DNA template strand is oriented from 3' to 5' for the RNA synthesis to occur from 5' to 3', this ensures that the synthesis progresses correctly in accordance with the base-pairing rules. Therefore, the correct answer is 3' to 5'.
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