Which method can be used to introduce a cloned vector into a bacterial cell A. transcription B. transformation C. transfection D.translation
Question
Which method can be used to introduce a cloned vector into a bacterial cell
A. transcription
B. transformation
C. transfection
D. translation
Solution
The method that can be used to introduce a cloned vector into a bacterial cell is B. Transformation.
Here's a step-by-step explanation:
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The first step in this process is to prepare the bacterial cells to take up the plasmid. This is done by making the cells competent, or able to take up DNA from the environment, through a process involving calcium chloride and heat shock.
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The plasmid DNA is then mixed with the competent cells. The mixture is briefly heated, which causes the bacteria to take up the plasmid.
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The bacteria are then placed on a medium that selects for the presence of the plasmid. Only bacteria that have taken up the plasmid will grow on this medium.
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The bacteria that grow on the medium are then tested to see if they have the correct plasmid. This can be done by checking for the presence of a marker gene that is on the plasmid.
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Once the bacteria with the correct plasmid have been identified, they can be grown up in large quantities for further study or use.
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