How much primary antibody should be added to prepare 14 mL solution of primary antibody with a dilution of 1:500?14 µL2.8 µL1.4 µL28 µL
Question
Solution 1
To prepare a 14 mL solution of primary antibody with a dilution of 1:500, you need to add 28 µL of primary antibody.
Here's the step-by-step calculation:
A 1:500 dilution means 1 part of the primary antibody is mixed with 499 parts of the diluent to make a total of 500 parts.
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