How much primary antibody should be added to prepare 14 mL solution of primary antibody with a dilution of 1:500?14 µL2.8 µL1.4 µL28 µL
Question
How much primary antibody should be added to prepare 14 mL solution of primary antibody with a dilution of 1:500?
- 14 µL
- 2.8 µL
- 1.4 µL
- 28 µL
Solution
To prepare a 14 mL solution of primary antibody with a dilution of 1:500, you need to add 28 µL of primary antibody.
Here's the step-by-step calculation:
-
A 1:500 dilution means 1 part of the primary antibody is mixed with 499 parts of the diluent to make a total of 500 parts.
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To find out how much primary antibody you need for 14 mL (or 14,000 µL, since 1 mL = 1,000 µL), you divide 14,000 µL by 500.
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14,000 µL / 500 = 28 µL
So, you need 28 µL of primary antibody.
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