Plot graph of the provide results and calculate the concentration and yield of bovine IgG in peak fraction.

To plot the graph and calculate the concentration and yield of bovine IgG in the peak fraction, it’s essential to follow a clear and structured approach. However, since specific numerical data is not provided in your request, I'll outline a general method you can apply to achieve this task.

1. Break Down the Problem

1. Identify data points for the peak fractions of bovine IgG (concentration values over time or volume).
2. Calculate the concentration of bovine IgG in the peak fraction.
3. Calculate the yield based on the total amount recovered.

2. Relevant Concepts

1. Concentration Calculation: If C is the concentration of IgG in mg/mL, and V is the volume of the peak fraction in mL: $\text{Concentration} = C = \frac{\text{mass of IgG}}{V}$

2. Yield Calculation: If Yield is defined as the ratio of the amount recovered to the initial amount loaded: $\text{Yield} (\%) = \left(\frac{\text{amount of IgG recovered}}{\text{initial amount of IgG}}\right) \times 100$

3. Analysis and Detail

1. Data Points: Gather your peak fraction data, which should include the concentration of bovine IgG and the corresponding volumes.
2. Calculations:
   • Calculate the total mass of IgG in the peak fraction.
   • Use the concentration and the volume of the peak fraction to ascertain the yield.

4. Verify and Summarize

1. Verification: Ensure the calculation for both concentration and yield are carefully reviewed.
2. Summary: The concentration gives an indication of how much bovine IgG is present in the peak fraction, while the yield tells us about the efficiency of the isolation or purification process.

Final Answer

While specific calculations cannot be performed without data, you can use the formulas above to compute the concentration and yield based on your own datasets. After calculating, plot the graph of your data points using software like Excel or Python (with libraries like Matplotlib) to visually represent the results. Make sure your graph labels are clear, indicating concentration on one axis and the corresponding volume or fraction on the other.