Sure, here are the steps to perform antibiotic susceptibility tests on cultured isolates to assess resistance patterns:

1. **Isolation of Bacteria**: The first step is to isolate the bacteria from the sample. This is usually done by streaking the sample on a nutrient agar plate and incubating it at a suitable temperature for the bacteria to grow.

2. **Preparation of Antibiotic Discs**: While the bacteria are growing, prepare antibiotic discs. These are small discs impregnated with specific concentrations of antibiotics. You can either purchase pre-made discs or make your own.

3. **Inoculation of Bacteria**: Once the bacteria have grown into colonies, pick a single colony and inoculate it into a liquid medium. This is to ensure that the bacteria you are testing are pure and not mixed with other species.

4. **Standardization of Bacterial Suspension**: The bacterial suspension should be standardized, usually to a 0.5 McFarland standard. This ensures that the same amount of bacteria is used in each test.

5. **Application of Antibiotic Discs**: The antibiotic discs are then applied to the surface of an agar plate that has been inoculated with the standardized bacterial suspension.

6. **Incubation**: The plates are then incubated, usually overnight, at a suitable temperature for the bacteria to grow.

7. **Measurement of Zones of Inhibition**: After incubation, the zones of inhibition around the antibiotic discs are measured. The size of the zone of inhibition indicates the susceptibility of the bacteria to the antibiotic. Larger zones indicate that the bacteria are more susceptible to the antibiotic.

8. **Interpretation of Results**: The results are then interpreted based on standard charts that indicate whether the bacteria are resistant, intermediate, or susceptible to each antibiotic.

9. **Reporting**: Finally, the results are reported to the clinician, who can use this information to choose the most effective antibiotic for treatment.

Question

Sure, here are the steps to perform antibiotic susceptibility tests on cultured isolates to assess resistance patterns:

1. **Isolation of Bacteria**: The first step is to isolate the bacteria from the sample. This is usually done by streaking the sample on a nutrient agar plate and incubating it at a suitable temperature for the bacteria to grow.

2. **Preparation of Antibiotic Discs**: While the bacteria are growing, prepare antibiotic discs. These are small discs impregnated with specific concentrations of antibiotics. You can either purchase pre-made discs or make your own.

3. **Inoculation of Bacteria**: Once the bacteria have grown into colonies, pick a single colony and inoculate it into a liquid medium. This is to ensure that the bacteria you are testing are pure and not mixed with other species.

4. **Standardization of Bacterial Suspension**: The bacterial suspension should be standardized, usually to a 0.5 McFarland standard. This ensures that the same amount of bacteria is used in each test.

5. **Application of Antibiotic Discs**: The antibiotic discs are then applied to the surface of an agar plate that has been inoculated with the standardized bacterial suspension.

6. **Incubation**: The plates are then incubated, usually overnight, at a suitable temperature for the bacteria to grow.

7. **Measurement of Zones of Inhibition**: After incubation, the zones of inhibition around the antibiotic discs are measured. The size of the zone of inhibition indicates the susceptibility of the bacteria to the antibiotic. Larger zones indicate that the bacteria are more susceptible to the antibiotic.

8. **Interpretation of Results**: The results are then interpreted based on standard charts that indicate whether the bacteria are resistant, intermediate, or susceptible to each antibiotic.

9. **Reporting**: Finally, the results are reported to the clinician, who can use this information to choose the most effective antibiotic for treatment.

Knowee AI · Accepted Answer