TLC is based on the principle of ---------------------a.None of the aboveb.Partition chromatographyc.Adsorption chromatographyd.Both Adsorption and Partition
Question
TLC is based on the principle of
- a. None of the above
- b. Partition chromatography
- c. Adsorption chromatography
- d. Both Adsorption and Partition
Solution
Thin Layer Chromatography (TLC) is primarily based on the principle of partition chromatography and can also involve aspects of adsorption chromatography.
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Partition Chromatography: In TLC, the stationary phase (the material coated on the plate) and the mobile phase (the solvent) allow for the separation of components based on their distribution between these two phases.
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Adsorption Chromatography: While TLC typically operates through partitioning, adsorption processes may also play a role when the stationary phase interacts with the compounds based on their polarity or affinity to the stationary phase.
Final Answer
Hence, the correct option for the principle on which TLC is based is d. Both Adsorption and Partition.
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