What 2 distances are important when calculating Rf values in chromatography?1Distance travelled by 2Distance travelled by solvent

The two distances that are important when calculating Rf values in chromatography are:

1. Distance travelled by the solute (the substance being separated): This is the distance from the origin (where the sample was initially applied) to the center of the spot where the solute ended up.

2. Distance travelled by the solvent (the substance used to move the solute): This is the distance from the origin to the final level reached by the solvent.

The Rf value (Retention Factor) is then calculated by dividing the distance travelled by the solute by the distance travelled by the solvent. This value helps to identify the solute, as each compound has a characteristic Rf value under specific conditions.