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What is the difference between a total-ion chromatogram and a mass chromatogram?

Question

What is the difference between a total-ion chromatogram and a mass chromatogram?

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Solution

A total-ion chromatogram (TIC) and a mass chromatogram (MC) are both outputs from a gas chromatography-mass spectrometry (GC-MS) analysis, but they represent different aspects of the data.

  1. Total-Ion Chromatogram (TIC): This is a plot of the total ion signal (intensity) detected by the mass spectrometer over time. It is essentially a sum of all the ions detected at each point in time. The TIC gives an overview of all components present in the sample, but it does not provide information about the specific mass-to-charge ratio (m/z) of the ions.

  2. Mass Chromatogram (MC): This is a plot of the ion signal (intensity) of a specific m/z value over time. It is essentially a trace of a specific ion or group of ions. The MC provides information about the presence and abundance of specific ions, which can be used to identify and quantify specific components in the sample.

In summary, the TIC provides a broad overview of the sample, while the MC provides detailed information about specific ions.

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