Which of the following matrix is not used while immobilizing the enzyme by covalent binding?*a) Agaroseb) Cellulosec) Calcium alginated) Glutaraldehyde

To determine which matrix is not typically used for immobilizing enzymes by covalent binding, we should analyze each option:

1. Agarose: This is a common matrix used for enzyme immobilization due to its porous nature, which allows for enzyme activity while providing a stable network for covalent binding.

2. Cellulose: Cellulose is also a commonly used support material for enzyme immobilization, as it can be chemically modified to facilitate covalent bond formation with enzymes.

3. Calcium alginate: This polysaccharide forms gels that can encapsulate enzymes, but it is more commonly associated with ionic gelation rather than covalent binding.

4. Glutaraldehyde: This is a chemical reagent used to create covalent bonds between enzymes and support matrices. It is often used to cross-link enzymes to a solid support.

Conclusion:

Calcium alginate is typically not used while immobilizing enzymes by covalent binding; it is primarily utilized for physical entrapment or ionic gelation.

Final Answer

c) Calcium alginate