What will happen if the transformed cells do not grow in the presence of the chemical inducer?

If the transformed cells do not grow in the presence of the chemical inducer, it could mean several things:

1. The transformation process may not have been successful: The cells may not have incorporated the foreign DNA that confers resistance to the chemical inducer. This could be due to issues with the transformation protocol, such as incorrect temperatures or timings, or problems with the DNA itself, such as degradation or incorrect preparation.

2. The inducer may be toxic to the cells: If the chemical inducer is toxic, the cells will not be able to grow even if they have been successfully transformed. This could be tested by attempting to grow non-transformed cells in the presence of the inducer.

3. The cells may not be expressing the resistance gene: Even if the cells have incorporated the foreign DNA, they may not be expressing the gene that confers resistance to the chemical inducer. This could be due to issues with the promoter region of the gene, or other regulatory elements that control gene expression.

4. The resistance gene may not be functional: If the gene that is supposed to confer resistance to the chemical inducer is not functional, the cells will not be able to grow in the presence of the inducer. This could be due to mutations in the gene, or problems with the protein that it encodes.

In order to determine the exact reason, further tests would need to be carried out, such as PCR to confirm the presence of the foreign DNA, or sequencing to check for mutations in the resistance gene.