State the role that the following factors play in gene cloning:Restriction enzymeLigasePlasmidVector

Restriction Enzyme: These are proteins that cut DNA at specific sequences. They are used in gene cloning to cut the DNA at the desired location. This allows for the insertion of the gene of interest into the vector.

Ligase: This enzyme is used to join two pieces of DNA together. In gene cloning, it is used to seal the gaps between the vector DNA and the inserted gene, creating a stable and continuous DNA molecule.

Plasmid: A plasmid is a small, circular piece of DNA that is separate from the chromosomal DNA. In gene cloning, plasmids are often used as vectors because they can replicate independently of the chromosomal DNA, allowing for the production of many copies of the gene of interest.

Vector: A vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed. In gene cloning, the vector (often a plasmid) is used to introduce the gene of interest into a host organism.