During a miniprep you are asked not to vortex your sample once you have added the lysis buffer. If you did what would be the outcome? Can you explain why?
Question
Solution 1
Vortexing the sample after adding the lysis buffer during a miniprep can lead to shearing of the genomic DNA, which can result in lower yield and quality of plasmid DNA.
Here's why:
- The lysis buffer contains a detergent that disrupts the cell membrane, releasing the cell's contents, including t Knowee AI is a powerful AI-powered study tool designed to help you to solve study problem.
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