ELISA is used to detect viruses where the key reagent isARNaseBAlkaline phosphataseCCatalaseDDNA probe

Understanding ELISA

ELISA (Enzyme-Linked Immunosorbent Assay) is a commonly used laboratory technique for detecting and quantifying proteins, antibodies, hormones, and viruses. It utilizes specific antibodies that bind to the target antigen, producing a measurable signal.

Key Reagent in ELISA

Among the options provided:

1. ARNase
2. Alkaline phosphatase
3. Catalase
4. DNA probe

Analysis of Each Option

1. ARNase: This enzyme is involved in degrading RNA and is not typically utilized in virus detection within ELISA.

2. Alkaline phosphatase: This is an enzyme commonly used in ELISA assays as a conjugate. It generates a measurable signal when a substrate is added, indicating the presence of the target antigen or antibody.

3. Catalase: While important in cellular processes to decompose hydrogen peroxide, it does not have a role in the detection of viruses in the ELISA format.

4. DNA probe: Usually used in nucleic acid-based tests rather than traditional ELISA; it helps detect specific sequences of DNA.

Conclusion

The most relevant reagent used in ELISA for detecting viruses among the options is Alkaline phosphatase (Option B) due to its role as a conjugate enzyme that provides the measurable signal in the assay.

Final Answer

B. Alkaline phosphatase is the key reagent used in ELISA to detect viruses.