Which of the following enzymes is involved in relieving DNA supercoiling during DNA replication?

The enzyme involved in relieving DNA supercoiling during DNA replication is called Topoisomerase.

Here are the steps explaining its role:

1. DNA replication involves the unwinding of the double helix structure of the DNA molecule. This process is initiated by an enzyme called DNA helicase, which breaks the hydrogen bonds between the base pairs, causing the two strands to separate.

2. This unwinding and separating of the DNA strands creates tension further along the DNA molecule, causing it to become overwound or 'supercoiled'.

3. Topoisomerase is the enzyme that comes into play here. It works to relieve this tension caused by supercoiling.

4. Topoisomerase cuts one or both strands of the DNA molecule, allowing it to unwind and relieve the tension, and then reseals