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Which of the following property of a protein is exploited in gel filtration chromatography

Question

Which of the following property of a protein is exploited in gel filtration chromatography?

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Solution

In gel filtration chromatography, the property of a protein that is exploited is its size or molecular weight.

Here's a step-by-step explanation:

  1. Gel filtration chromatography, also known as size exclusion chromatography, separates proteins based on their size.

  2. The chromatography column is packed with gel-like beads that have tiny pores. These pores are of a specific size.

  3. When a mixture of proteins is passed through the column, smaller proteins enter the pores and take longer to pass through the column.

  4. Larger proteins, on the other hand, cannot enter the pores and thus travel through the column more quickly.

  5. In this way, proteins are separated based on their size, with larger proteins eluting from the column before smaller ones.

So, the property of a protein that is exploited in gel filtration chromatography is its size or molecular weight.

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