A protein was eluted at 15 ml when passed through a gel filtration column with a void volume of 5 ml and a pore volume of 1 ml. The Kd value of the protein is
Question
Solution 1
The Kd value, or partition coefficient, is a measure of how a protein distributes itself between the mobile phase and stationary phase in chromatography. It can be calculated using the formula:
Kd = (Ve - V0) / (Vt - V0)
where: Ve = elution volume (the volume at which the protein comes off the col Knowee AI StudyGPT is a powerful AI-powered study tool designed to help you to solve study prob
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