Which of the following methods would be best for sterilizing a sample of a heat-sensitive solution?ANSWERUV radiationAutoclavingFiltrationFreezing

To determine the best method for sterilizing a heat-sensitive solution, let's analyze the options provided:

1. UV Radiation: This method uses ultraviolet light to kill bacteria and other microorganisms. It is effective for surfaces and air but may not penetrate well into liquids, making it less ideal for sterilizing heat-sensitive solutions.

2. Autoclaving: This method involves using high-pressure steam and temperatures (usually around 121°C) to sterilize. However, this method is not suitable for heat-sensitive solutions as it may denature proteins or alter the chemical structure of the solution.

3. Filtration: This technique physically separates microorganisms from the solution by passing it through a membrane filter with pores small enough to trap bacteria. It is ideal for heat-sensitive solutions since it does not involve heat.

4. Freezing: While freezing can inhibit the growth of some microorganisms, it does not effectively sterilize a solution. Some microbes can survive freezing, and it may not eliminate all pathogens.

Conclusion:

Among the options given, filtration is the most appropriate method for sterilizing a heat-sensitive solution, as it effectively removes microorganisms without the use of heat.