In which period was the system of rule over the Roman Empire first divided between Rome and Constantinople?301–400 CE200–100 BCE401–500 CE101–200 CE100–1 BCE

The correct answer is 401–500 CE.

Explanation:

The Roman Empire was officially divided into Eastern and Western regions during the reign of Emperor Theodosius I, who ruled from 379 to 395 CE. After his death in 395 CE, the empire was permanently divided between his two sons, Arcadius and Honorius, with Arcadius ruling the Eastern Roman Empire (Constantinople) and Honorius governing the Western Roman Empire (Rome). This division marked the beginning of a distinct separation between the Eastern and Western parts of the empire, leading to differing political and cultural developments. The period 401–500 CE thus encapsulates the time frame when this division was fully established.