Which special type of PCR would be used to detect RNA in a sample?ANSWERReal-time PCRTraditional PCR is sufficient.RT-PCRqPCR
Question
Which special type of PCR would be used to detect RNA in a sample?
- ANSWER
- Real-time PCR
- Traditional PCR is sufficient.
- RT-PCR
- qPCR
Solution
To detect RNA in a sample, the correct method to use is RT-PCR (Reverse Transcription Polymerase Chain Reaction).
Explanation:
- RT-PCR starts with the reverse transcription of RNA into complementary DNA (cDNA) using the enzyme reverse transcriptase. This cDNA can then be amplified using conventional PCR techniques.
- Real-time PCR (qPCR) is often used in conjunction with RT-PCR to quantify the cDNA produced during the PCR process in real-time, but it specifically measures DNA amplification, not RNA directly.
- Traditional PCR is primarily designed to amplify DNA and would not be effective for RNA detection without the reverse transcription step.
Therefore, for the detection of RNA, RT-PCR is specifically designed for that purpose.
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