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Which special type of PCR would be used to detect RNA in a sample?ANSWERReal-time PCRTraditional PCR is sufficient.RT-PCRqPCR

Question

Which special type of PCR would be used to detect RNA in a sample?

  • ANSWER
    • Real-time PCR
    • Traditional PCR is sufficient.
    • RT-PCR
    • qPCR
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Solution

To detect RNA in a sample, the correct method to use is RT-PCR (Reverse Transcription Polymerase Chain Reaction).

Explanation:

  1. RT-PCR starts with the reverse transcription of RNA into complementary DNA (cDNA) using the enzyme reverse transcriptase. This cDNA can then be amplified using conventional PCR techniques.
  2. Real-time PCR (qPCR) is often used in conjunction with RT-PCR to quantify the cDNA produced during the PCR process in real-time, but it specifically measures DNA amplification, not RNA directly.
  3. Traditional PCR is primarily designed to amplify DNA and would not be effective for RNA detection without the reverse transcription step.

Therefore, for the detection of RNA, RT-PCR is specifically designed for that purpose.

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