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Which of these enzymes is used in a PCR? RNA polymerase EcoRI DNA polymerase DNA ligase DNA reverse transcriptase

Question

Which of these enzymes is used in a PCR?

  • RNA polymerase
  • EcoRI
  • DNA polymerase
  • DNA ligase
  • DNA reverse transcriptase
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Solution

The enzyme used in a PCR (Polymerase Chain Reaction) is DNA polymerase.

Here's a step-by-step explanation:

  1. Denaturation: The double-stranded DNA is heated to separate it into two single strands.

  2. Annealing: During the cooling process, primers are attached to each original strand of DNA.

  3. Extension: DNA polymerase then adds nucleotides to the 3' end of each primer, copying the original strands of DNA.

  4. The process is repeated, doubling the amount of DNA with each cycle.

So, the correct answer is DNA polymerase.

This problem has been solved

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