Which of these enzymes is used in a PCR? RNA polymerase EcoRI DNA polymerase DNA ligase DNA reverse transcriptase
Question
Which of these enzymes is used in a PCR?
- RNA polymerase
- EcoRI
- DNA polymerase
- DNA ligase
- DNA reverse transcriptase
Solution
The enzyme used in a PCR (Polymerase Chain Reaction) is DNA polymerase.
Here's a step-by-step explanation:
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Denaturation: The double-stranded DNA is heated to separate it into two single strands.
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Annealing: During the cooling process, primers are attached to each original strand of DNA.
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Extension: DNA polymerase then adds nucleotides to the 3' end of each primer, copying the original strands of DNA.
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The process is repeated, doubling the amount of DNA with each cycle.
So, the correct answer is DNA polymerase.
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