The fragments of DNA formed after treatment with endonucleases are separated by the technique of Gel Electrophoresis.

Here are the steps:

1. DNA samples are prepared: The DNA is cut into fragments using restriction enzymes (endonucleases).

2. Loading the DNA into the gel: The DNA fragments are loaded into wells in the gel.

3. Applying an electric current: The gel is placed in a buffer solution, and an electric current is applied. DNA is negatively charged, so it moves towards the positive electrode.

4. Separation of DNA fragments: Smaller DNA fragments move faster and therefore travel further than larger ones.

5. Visualization: After the electrophoresis is complete, the DNA is stained to make it visible. The DNA fragments appear as bands on the gel. Each band represents a specific size of DNA fragment.

Question

The fragments of DNA formed after treatment with endonucleases are separated by the technique of Gel Electrophoresis.

Here are the steps:

1. DNA samples are prepared: The DNA is cut into fragments using restriction enzymes (endonucleases).

2. Loading the DNA into the gel: The DNA fragments are loaded into wells in the gel.

3. Applying an electric current: The gel is placed in a buffer solution, and an electric current is applied. DNA is negatively charged, so it moves towards the positive electrode.

4. Separation of DNA fragments: Smaller DNA fragments move faster and therefore travel further than larger ones.

5. Visualization: After the electrophoresis is complete, the DNA is stained to make it visible. The DNA fragments appear as bands on the gel. Each band represents a specific size of DNA fragment.

Knowee AI · Accepted Answer

The fragments of DNA formed after treatment with endonucleases are separated by the technique of Gel Electrophoresis.

Here are the steps:

1. DNA samples are prepared: The DNA is cut into fragments using restriction enzymes (endonucleases).

2. Loading the DNA into the gel: The DNA fragments are loaded into wells in the gel.

3. Applying an electric current: The gel is placed in a buffer solution, and an electric current is applied. DNA is negatively charged, so it moves towards the positive electrode.

4. Separation of DNA fragments: Smaller DNA fragments move faster and therefore travel further than larger ones.

5. Visualization: After the electrophoresis is complete, the DNA is stained to make it visible. The DNA fragments appear as bands on the gel. Each band represents a specific size of DNA fragment.

Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______

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Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______

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