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Cryo preservation techniques involves by using*2 pointsLiquid sulphurLiquid NitrogenLiquid chlorineDry ice

Question

Cryo preservation techniques involves by using

  • Liquid sulphur
  • Liquid Nitrogen
  • Liquid chlorine
  • Dry ice
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Solution

Cryo preservation techniques involve the use of Liquid Nitrogen. Here are the steps:

  1. Sample Preparation: The biological material to be preserved is prepared. This could be cells, tissues, or even whole organisms. The preparation often involves placing the material in a solution that will protect it from damage during freezing and thawing.

  2. Cooling: The prepared sample is then cooled to very low temperatures, typically using liquid nitrogen. This is done slowly to prevent the formation of ice crystals which can damage the material.

  3. Storage: Once the sample has been cooled to the appropriate temperature, it is stored in a cryo preservation unit. This keeps the sample at a constant low temperature, typically -196 degrees Celsius, the boiling point of liquid nitrogen.

  4. Thawing: When the material is needed, it is carefully warmed up to room temperature. This is done slowly to prevent damage from rapid temperature changes.

  5. Recovery: After thawing, the material is often placed in a suitable environment to recover. For cells, this might be a nutrient-rich culture medium.

So, the correct answer is Liquid Nitrogen.

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