When an enzyme makes a single cut on a plasmid, it linearizes the plasmid. Here's the explanation of how this affects the bands seen on a gel electrophoresis:

1. **Understanding Plasmids**: Plasmids are typically circular DNA molecules. When they remain circular, they may run as a single band on an agarose gel.

2. **Effect of the Cut**: When an enzyme (restriction endonuclease) makes a single cut in the circular plasmid, it converts it into a linear form. This linearized plasmid will then migrate differently on the gel compared to the circular form.

3. **Result on Gel**: After running the gel, if the plasmid was originally a single circular plasmid, after one cut it will be in linear form, resulting in **one band** appearing on the gel.

### Final Answer
You will see **one band** on the gel after the enzyme makes a single cut on the plasmid.

Question

When an enzyme makes a single cut on a plasmid, it linearizes the plasmid. Here's the explanation of how this affects the bands seen on a gel electrophoresis:

1. **Understanding Plasmids**: Plasmids are typically circular DNA molecules. When they remain circular, they may run as a single band on an agarose gel.

2. **Effect of the Cut**: When an enzyme (restriction endonuclease) makes a single cut in the circular plasmid, it converts it into a linear form. This linearized plasmid will then migrate differently on the gel compared to the circular form.

3. **Result on Gel**: After running the gel, if the plasmid was originally a single circular plasmid, after one cut it will be in linear form, resulting in **one band** appearing on the gel.

### Final Answer
You will see **one band** on the gel after the enzyme makes a single cut on the plasmid.

Knowee AI · Accepted Answer

When an enzyme makes a single cut on a plasmid, it linearizes the plasmid. Here's the explanation of how this affects the bands seen on a gel electrophoresis:

1. **Understanding Plasmids**: Plasmids are typically circular DNA molecules. When they remain circular, they may run as a single band on an agarose gel.

2. **Effect of the Cut**: When an enzyme (restriction endonuclease) makes a single cut in the circular plasmid, it converts it into a linear form. This linearized plasmid will then migrate differently on the gel compared to the circular form.

3. **Result on Gel**: After running the gel, if the plasmid was originally a single circular plasmid, after one cut it will be in linear form, resulting in **one band** appearing on the gel.

### Final Answer
You will see **one band** on the gel after the enzyme makes a single cut on the plasmid.

If an enzyme makes a single cut on a plasmid, how many bands will you see on the gel?fourthreetwoone

Question

If an enzyme makes a single cut on a plasmid, how many bands will you see on the gel?

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