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The following enzyme(s) are used A. RNA polymerase B. Helicase, DNA polymerase as RNaseH C. Thermostable DNA polymerase D. DNA polymerase III

Question

The following enzyme(s) are used

A. RNA polymerase
B. Helicase, DNA polymerase as RNaseH
C. Thermostable DNA polymerase
D. DNA polymerase III

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Solution

The enzymes mentioned in your question are used in various biological processes:

A. RNA polymerase: This enzyme is involved in the process of transcription, where it synthesizes RNA from a DNA template.

B. Helicase, DNA polymerase, and RNaseH: These enzymes are involved in DNA replication. Helicase unwinds the DNA double helix, DNA polymerase synthesizes the new DNA strands, and RNaseH removes the RNA primers that initiate DNA synthesis.

C. Thermostable DNA polymerase: This enzyme is used in the Polymerase Chain Reaction (PCR) process, where it can withstand the high temperatures needed for DNA denaturation.

D. DNA polymerase III: This is the main enzyme involved in DNA replication in bacteria. It adds nucleotides to the new DNA strand at the replication fork.

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