Which of the following is a constitutive promotor in yeast expression system (a) GAPDH (b) AOX1 (c) MOX (d) AOX2
Question
Which of the following is a constitutive promoter in yeast expression system?
(a) GAPDH
(b) AOX1
(c) MOX
(d) AOX2
Solution
The constitutive promoter in a yeast expression system among the options provided is (a) GAPDH.
Here's a step-by-step explanation:
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A constitutive promoter is a type of promoter that is continuously active, meaning it is always "on" and transcribing the downstream gene.
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In the context of yeast expression systems, the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) promoter is known to be constitutive. This means that the GAPDH promoter is always active and continuously drives the expression of the gene it controls.
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On the other hand, AOX1 (Alcohol oxidase 1), MOX (Methanol oxidase), and AOX2 (Alcohol oxidase 2) are inducible promoters in yeast. They are not always active but can be turned "on" or "off" under certain conditions. For example, the AOX1 promoter is strongly induced by methanol.
So, the correct answer is (a) GAPDH.
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