Determining the iron hemoglobin content in a blood sample is crucial for diagnosing conditions like anemia. In an underdeveloped country where power supply is an issue, you can use a method that doesn't require electricity, such as the Sahli's method (also known as acid hematin method). Here's a step-by-step guide:

1. Collect the Blood Sample: The first step is to collect a blood sample. This is typically done by pricking the finger with a sterile lancet and collecting the blood in a capillary tube.

2. Prepare the Sahli's Hemoglobinometer: This device consists of a graduated glass tube, a comparator, and a standard colored glass. Fill the tube up to the mark 20 with 0.1N hydrochloric acid (HCl).

3. Add the Blood Sample: Add the blood sample to the tube until the level of the liquid reaches the mark 20. Mix the blood with the HCl by stirring with the provided stirrer.

4. Allow to Stand: Allow the tube to stand for about 10 minutes. This allows the blood to be converted to acid hematin, which is a brown-colored solution.

5. Dilute the Solution: Gradually add distilled water, while stirring, until the color of the solution matches that of the standard colored glass when viewed through the comparator.

6. Read the Hemoglobin Level: The level of the solution in the tube now corresponds to the hemoglobin level in the blood sample. This can be read off the scale on the tube.

This method provides a rough estimate of the hemoglobin level and is less accurate than other methods that require electricity, like the cyanmethemoglobin method. However, it can be useful in settings where resources are limited. It's important to note that this method should be carried out by a trained healthcare professional and the results should be interpreted in the context of the patient's overall health and symptoms.

Question

Determining the iron hemoglobin content in a blood sample is crucial for diagnosing conditions like anemia. In an underdeveloped country where power supply is an issue, you can use a method that doesn't require electricity, such as the Sahli's method (also known as acid hematin method). Here's a step-by-step guide:

1. Collect the Blood Sample: The first step is to collect a blood sample. This is typically done by pricking the finger with a sterile lancet and collecting the blood in a capillary tube.

2. Prepare the Sahli's Hemoglobinometer: This device consists of a graduated glass tube, a comparator, and a standard colored glass. Fill the tube up to the mark 20 with 0.1N hydrochloric acid (HCl).

3. Add the Blood Sample: Add the blood sample to the tube until the level of the liquid reaches the mark 20. Mix the blood with the HCl by stirring with the provided stirrer.

4. Allow to Stand: Allow the tube to stand for about 10 minutes. This allows the blood to be converted to acid hematin, which is a brown-colored solution.

5. Dilute the Solution: Gradually add distilled water, while stirring, until the color of the solution matches that of the standard colored glass when viewed through the comparator.

6. Read the Hemoglobin Level: The level of the solution in the tube now corresponds to the hemoglobin level in the blood sample. This can be read off the scale on the tube.

This method provides a rough estimate of the hemoglobin level and is less accurate than other methods that require electricity, like the cyanmethemoglobin method. However, it can be useful in settings where resources are limited. It's important to note that this method should be carried out by a trained healthcare professional and the results should be interpreted in the context of the patient's overall health and symptoms.

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