Adsorption' principle is used for which of the following purification method?ExtractionChromatographyDistillationSublimation
Question
Adsorption' principle is used for which of the following purification method?
- Extraction
- Chromatography
- Distillation
- Sublimation
Solution
The principle of 'Adsorption' is used in the purification method of Chromatography.
Here's a step-by-step explanation:
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In chromatography, a mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
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The various components of the mixture travel at different speeds, causing them to separate.
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The separation is based on differential partitioning between the mobile and stationary phases.
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Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.
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Adsorption, in this context, refers to the process by which molecules of the substance being purified (the solute) are taken up by the stationary phase.
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Different substances in the mixture will interact differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out of the column.
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