What component of the PCR is omitted in the reaction used as Negative control? Template DNA dNTPs DNA polymerase Primers Reaction Buffer

In a PCR (Polymerase Chain Reaction) setup, a negative control is used to ensure that no DNA contamination is affecting the results of the experiment. The component that is typically omitted in the negative control reaction is the Template DNA.

Explanation of the Components:

1. Template DNA: The starting material that contains the target sequence to be amplified. In a negative control, this is omitted to check for contamination; if there is amplification, it indicates that contamination may have occurred.

2. dNTPs (deoxynucleotide triphosphates): These are the building blocks for new DNA strands and are included in all PCR reactions, including the negative control.

3. DNA Polymerase: This enzyme synthesizes new DNA strands. It is also included in the negative control because it does not contribute to the specificity of the amplification.

4. Primers: Short sequences that initiate DNA synthesis are included in both the experimental and negative control reactions.

5. Reaction Buffer: This component maintains the optimal pH and ionic conditions for PCR, and it is present in all reactions.

Therefore, in the negative control reaction, the Template DNA is omitted.