Decreasing the ratio of acrylamide:bisacrylamide when pouring an SDS-PAGE gel makes the gel "thicker" (less porous). Can you explain why?
Question
Solution 1
Sure, I'd be happy to explain.
The ratio of acrylamide to bisacrylamide in an SDS-PAGE gel determines the porosity of the gel. Acrylamide is the primary component of the gel and forms long, linear polymers. Bisacrylamide, on the other hand, acts as a cross-linker between these polymers.
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