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Mannitol Salt Agar (MSA) differentiates among bacteria based on their ability to ferment mannitol.Group of answer choicesTrueFalse

Question

Mannitol Salt Agar (MSA) differentiates among bacteria based on their ability to ferment mannitol.

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  • False
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Solution

Answer:

True.

Mannitol Salt Agar (MSA) is a selective and differential culture medium that is primarily used for isolating staphylococci. It is selective due to its high salt concentration, which inhibits the growth of most bacteria other than staphylococci. Furthermore, MSA contains mannitol, a carbohydrate that some staphylococci can ferment. When mannitol is fermented, it produces acid, which leads to a color change in the pH indicator present in the medium (phenol red). This property allows for differentiation between mannitol-fermenting bacteria, such as Staphylococcus aureus, which produces yellow colonies, and non-fermenting species, which retain the red color of the medium. Thus, MSA effectively differentiates among bacteria based on their ability to ferment mannitol.

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