The pI of a protein is 6.5. Which of the following buffers should be used so that the protein could bind to a cation exchange column for purification
Question
Solution 1
To bind a protein to a cation exchange column, the protein must have a positive charge. The charge of a protein is determined by the pH of the solution it is in relative to the protein's isoelectric point (pI). At a pH below its pI, a protein will carry a net positive charge.
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Knowee AI StudyGPT is a powerful AI-powered study tool designed to help you to solve study problem.
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