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Molecular cloning for in vitro transcription. Select the palindrome sequence(s).

Question

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Solution

To identify palindrome sequences in the context of molecular cloning and in vitro transcription, we should consider that a palindrome in DNA is a sequence that reads the same in both directions (5' to 3' and 3' to 5'). Common examples are restriction enzyme recognition sites, which are often palindromic.

Palindrome sequences typically exhibit a symmetrical structure. Here are a few well-known palindromic sequences:

  1. EcoRI: 5' - GAATTC - 3'
    3' - CTTAAG - 5'

  2. HindIII: 5' - AAGCTT - 3'
    3' - TTCGAAC - 5'

  3. BamHI: 5' - GGATCC - 3'
    3' - CCTAGG - 5'

To find more specific palindromic sequences within a given context or sequence, you'll need to analyze the specific nucleotides provided in that context.

If you have a specific sequence or context in mind, please share it for a more tailored identification of palindrome sequences.

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