which is the most initial step to separate water soluble proteins from insoluble cellular material is

The most initial step to separate water soluble proteins from insoluble cellular material is to perform a cell lysis procedure. This involves breaking open the cells to release their contents. Here are the steps involved in the cell lysis process:

1. Harvest the cells: Start by growing the cells of interest in a suitable culture medium. Once the cells have reached the desired density, harvest them by centrifugation or filtration.

2. Wash the cells: After harvesting, wash the cells with a buffer solution to remove any contaminants or media components.

3. Resuspend the cells: Resuspend the cells in a lysis buffer that contains detergents and protease inhibitors. The lysis buffer helps to disrupt the cell membrane and solubilize the proteins.

4. Incubate the cells: Allow the cells to incubate in the lysis buffer for a specific period of time. This allows the detergents to permeate the cell membrane and break it down, releasing the cellular contents.

5. Disrupt the cells: Apply mechanical disruption methods such as sonication, homogenization, or freeze-thaw cycles to further break open the cells and release the proteins.

6. Centrifuge the lysate: Centrifuge the lysate at high speed to separate the insoluble cellular material, such as cell debris and membranes, from the soluble proteins. The centrifugation step will pellet the