Which molecule serves as the precursor for the synthesis of carbamoyl phosphate in the de novo biosynthesis of pyrimidine nucleotides?
Question
Which molecule serves as the precursor for the synthesis of carbamoyl phosphate in the de novo biosynthesis of pyrimidine nucleotides?
Solution
The molecule that serves as the precursor for the synthesis of carbamoyl phosphate in the de novo biosynthesis of pyrimidine nucleotides is bicarbonate (HCO3-) and ammonia (NH3).
Here are the steps:
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The first step in the synthesis of carbamoyl phosphate is the activation of bicarbonate by ATP to form carboxyphosphate and ADP in a reaction catalyzed by the enzyme carbamoyl phosphate synthetase.
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In the second step, ammonia reacts with carboxyphosphate to form carbamic acid and inorganic phosphate (Pi). This reaction is also catalyzed by carbamoyl phosphate synthetase.
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In the third and final step, carbamic acid is phosphorylated by ATP to form carbamoyl phosphate and ADP, again catalyzed by the enzyme carbamoyl phosphate synthetase.
So, the precursor molecules for the synthesis of carbamoyl phosphate are bicarbonate and ammonia.
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